Current Issue : April - June Volume : 2010 Issue Number : 1 Articles : 6 Articles
A simple high-performance thin-layer chromatographic (HPTLC) method has been developed and validated for the quantitative determination of silybin, a flavanol, which is the major active constituent of pharmacologically important favanolignane group, silymarin in Silybum marianum L and its homeopathic mother tincture. The method involves the separation and quantification of the analyte on silica gel 60 GF254 HPTLC plates with visualisation under UV and scanning at 290 nm. Using this technique, the silybin content of the seeds of the said plant and its homeopathic mother tincture has been determined....
A particle size distribution method was developed and validated for rabeprazole sodium by using laser diffraction technique. The determination of the particle size was carried out by using wet measurements. The dispersant consisted of light liquid paraffin oil with a refractive index as 1.430. The particle refractive index was set at 1.54 and stirrer speed was set at 2000 rpm with absorption value of 0.1 and obscuration of 15%. The developed method was further validated statistically by evaluating the instrumental parameters like stirrer speed, obscuration, particle refractive index, dispersant refractive index, sonication time, volume of dispersant, vortex time, sample concentration and validation parameter like repeatability. The particle size distribution i.e. diameter of 90% particles d(0.9) of rabeprazole was found to be < 50 µm. The method has been successfully used to analyze active pharmaceutical ingredient rabeprazole sodium with good reproducibility....
The term “hydrotropy” has been used to designate the increase in aqueous solubility of various poorly water-soluble compounds due to the presence of large amount of additives. Various hydrotropic agents have been used to enhance the aqueous solubility of a large number of drugs. Sodium benzoate, sodium salicylate, niacinamide, and urea have been employed to enhance the aqueous solubility of poorly water-soluble drugs. Various organic solvents like methanol, chloroform, ethanol, benzene, hexane, acetone, diethyl ether and acetonitrile are widely used in spectrophotometric estimations of poorly water-soluble drugs. Most of these organic solvents are toxic, costlier and sources of pollution. Inaccuracy in spectrophotometric estimations due to volatility of organic solvents is another drawback of these solvents. A novel, safe and sensitive method of spectrophotometric estimation in ultraviolet region is developed using 7.5 M N,N’-Dimethylurea .solution for the quantitative determination of metronidazole (poorly water-soluble drug) in tablet dosage form. There was more than 4 times enhancement in the aqueous solubility of metronidazole in 7.5M N’Ndimethyl urea solution (an inexpensive chemical used here as solubilizing hydrotropic agent). Metronodazole shows maximum absorbance at 320 nm. N’Ndimethyl urea sodium does not absorb above 260 nm. Results of the analysis were validated statistically and by recovery studies. Using 7.5 M N’Ndimethyl urea solution for analysis of two different tablet formulations of metronidazole, the percent label claims and recoveries estimated were close to 100 with low values of standard deviation, percent coefficient of variation and standard error. Thus method is ecofriendly, less toxic, inexpensive more accurate....
Simple, rapid and novel potentiometric method is developed for the determination of nifedipine in tablets. The proposed method is based on the reduction of the nitro group to amino group of the molecule with zinc and hydrochloric acid. The reduced molecule was diazotised with sodium nitrite at 5-10 oC. The reactions were carried out in aqueous medium without use of organic solvents. This method was validated statistically by low values of statistical parameters viz, standard deviation, relative standard deviation and standard error of mean. This method is novel, economic and eco-friendly as the method does not involve the use of any organic solvent. The proposed method is recommended for quality control, routine analysis and developed for academic purpose....
A reverse phase high performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of atorvastatin and ramipril in marketed formulation is developed. The determination was carried out on a HIQ SII C18 column -10 (4.5 mm x 250 mm).column using a mobile phase of acetonitrile: 0.02 M Potassium dihydrogen phosphate (pH 3.2) [75: 25%v/v]. The flow rate was 1 ml/min with detection at 217 nm. The Valsartan is used as internal standard in this method. The retention time for atorvastatin was 6.1 min, for ramipril 3.43 min. Atorvastatin and ramipril showed a linear response in the concentration range of 20-180 μg/ml and 10-90 μg/ml respectively. The correlation co-efficient (‘r’ value) for atorvastatin and ramipril was 0.9999 for both drugs. The results of analysis have been validated statistically and by recovery studies. The percentage recoveries obtained for atorvastatin and ramipril ranges from 99.04 to 101.15 %....
Two simple, economical, precise and reproducible visible spectrophotometric methods have been developed for the estimation of Dicycloverine Hydrochloride. The developed methods are based on the formation of chloroform extractable complex of Dicycloverine hydrochloride with bromo phenol blue and phenol red. The complex with bromo phenol blue (method I) shows absorbance maxima at 387.4 nm and linearity in the concentration range of 10-60 µg/ml. The extracted complex with phenol red (Method II) shows absorbance maxima at 373.4 nm and the linearity in the concentration range of 10-80 µg/ml. Results of analysis for both the methods were validated statistically and by recovery studies. These methods were successively applied to pharmaceutical formulation because no interferences from tablet excipients were found. The suitability of these methods for the quantitative determination of the compounds was proved by validation....
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